Sampling methods airborne viruses
Any microorganism, including viruses, can become airborne. Contaminated material can be aerosolized in many different ways, ranging from wind to human and animal activities such as sneezing, mechanical processes, etc. If the aerodynamic size of an infectious particle is appropriate, it can remain airborne, come into contact with humans or animals, and potentially cause an infection. The probability of an airborne microorganism-laden particle causing an infection depends on its infectious potential and its ability to resist the stress of aerosolization.
Airborne microorganisms can represent major health and economic risks to human and animal populations. Appropriate preventive actions can be taken if the threat posed by such microorganisms is better understood. Authorities need to be aware of the nature, concentration, and pathogenicity of airborne microorganisms to better control them. This information can be obtained by using various air sampling methods, each of which has its particular advantages and disadvantages. Many types of samplers and analytical methods have been used over the years.
Most air sampling technologies depend on the aerodynamic diameter of the airborne particles, the adhesion properties of airborne particles, Brownian motion, thermal gradients, and the inertia of the particles. Aerosolized particles attach to any surface with which they come into contact. Adhesive forces such as van der Waals forces, electrostatic forces, and surface tension partly explain this adhesion. Most of the sampling methodologies presented in this review are based on this principle.
Airborne particles with aerodynamic diameters on the order of 100 nm or less are prone to a particular way of moving, mainly due to the billions of collisions they encounter with gas molecules. This is called Brownian motion, and the smaller the particle, the greater the movement and the more likely that the particle will diffuse, come into contact with a surface, and adhere to it. When this happens, the other suspended particles occupy the space left vacant by the particle that has adhered to the surface. This phenomenon is the basis for the efficient removal of very small particles by filtration, particularly when the distance between two surfaces of the filter is sufficient for the particles to pass through.
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Nicola B
Editorial Team
Journal of Biochemistry and Biotechnology